So the plotting concentration on horizontal and Kc/R(θ), which is equivalent as a reciprocal number of scattering intensity, on vertical after measuring scattering intensities of variable concentrations is called Debye Plot.Since angle dependency of scattering intensity appears in the large molecular weight sample, measuring scattering intensity at variable angle(θ) provides more accuracy on molecular weight measurement and radium of gyration, which is yardstick of molecular dispersion.When measured at fixed angle, inputting expected radium of gyration, correcting as an angle dependent measurement, accuracy of molecular weight is improved. Dynamic light scattering (DLS) is a very popular technique for the measurement of particle size distributions in colloidal suspensions and emulsions. In reality, scattering intensity to be obtained depends upon concentration, too. Dynamic light scattering particle size analyzers are used to measure the size of very small particles (0.6 nm to 6 um) in solution. Electrophoretic light scattering method was utilized for zeta potential measurement. The Z-Ave value was reported as the mean diameter of nanoparticles where the cumulant method was adopted for data analysis 19. To simplify, from the bigger particle gives the stronger scattering and the smaller particle gives weaker scattering. Particle size was measured using dynamic light scattering method where the scattered light was collected at 173°. Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. As a principle, molecular weight is calculated from absolute value of scattered light obtained by irradiated light into colloidal particle. Static Light Scattering Method is renowned as the convenient method to know absolute molecular weight. Figure 1: Exemplary correlation function of a DLS measurement. Molecular Weight: Static Light Scattering Method The particle size range of DLS depends on the properties of the analyzed species, such as refractive index or density, as well as the surrounding formulation. The median (D 50) should be considered to get a more accurate average particle size Dynamic light scattering (DLS) results DLS measurements provide results as the hydrodynamic diameter (HDD) and peak size (see Figure 1 and 2).
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